Enzima restrictiva de clasa I Fse I

Caracteristici standard:
Specificatii complete
Product Name FseI
Concentration:  2,000 units/ml
Unit Definition: One unit is defined as the amount of enzyme required to digest 1 µg of pBC4 DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
Shelf Life: 12 months
Storage Temp:  -80 °C
Storage Conditions: 200 mM NaCl, 10 mM Tris-HCl (pH 7.4), 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 200 µg/ml BSA
Specification Version: PS-R0588S/L v2.0
Effective Date: 17-Dec-13
Endonuclease Activity (Nicking) - A 50 µl reaction in CutSmart™ Buffer containing 1 µg of supercoiled PhiX174 DNA and a minimum of 10 Units of FseI incubated for 4 hours at 37ºC results in
Exonuclease Activity (Radioactivity Release) - A 50 µl reaction in CutSmart™ Buffer containing 1 µg of a mixture of single and doublestranded [ ³H] E. coli DNA and a minimum of 10 units of FseI incubated for 4 hours at 37ºC releases
Ligation and Recutting (Terminal Integrity) - After a 10-fold over-digestion of pBC4 DNA with FseI, >95% of the DNA fragments can be ligated with T4 DNA ligase in 16 hours at 16ºC. Of these ligated fragments, >95% can be recut with FseI.
Non-Specific DNase Activity (16 Hour) - A 50 µl reaction in CutSmart™ Buffer containing 1 µg of pBC4 DNA and a minimum of 10 units of FseI incubated for 16 hours at 37ºC results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis
Protein Purity Assay (SDS-PAGE) - FseI is >95% pure as determined by SDS PAGE analysis using Coomassie Blue detection.