Enzima restrictiva de clasa II Hpy166 II

Caracteristici standard:
Specificatii complete
Product Name Hpy166II
Concentration:  10,000 units/ml
Unit Definition: One unit is defined as the amount of enzyme required to digest 1 Ƚg of pBR322 in 1 hour at 37°C in total reaction volume of 50 Ƚl.
Shelf Life: 24 months
Storage Temp: -20 °C
Storage Conditions: 250 mM NaCl, 10 mM Tris-HCl (pH 7.4), 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.15% Triton X-100, 200 µg/ml BSA
Specification Version: PS-R0616S/L v1.0
Effective Date: 10-Oct-13
Exonuclease Activity (Radioactivity Release) - A 50 µl reaction in CutSmart™ Buffer containing 1 µg of a mixture of single and doublestranded [ ³H] E. coli DNA and a minimum of 30 units of Hpy166II incubated for 4 hours at 37ºC releases
Ligation and Recutting (Terminal Integrity) - After a 10-fold over-digestion of pBR322 DNA with Hpy166II, >95% of the DNA fragments can be ligated with T4 DNA ligase in 16 hours at 16ºC. Of these ligated fragments, >95% can be recut with Hpy166II.
Non-Specific DNase Activity (16 Hour) - A 50 µl reaction in CutSmart™ Buffer containing 1 µg of pBR322 DNA and a minimum of 50 units of Hpy166II incubated for 16 hours at 37ºC results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis.
Protein Purity Assay (SDS-PAGE) - Hpy166II is >95% pure as determined by SDS PAGE analysis using Coomassie Blue detection.