Testul HALOTECH de determinare a fragmentării ADN din spermă

Tradiționala spermogramă nu ia în considerare cel mai important factor- calitatea materialului genetic din celulele spermatice, ajutând la alegerea celei mai bune metode de reproducere artificială, compatibilitatea donatorilor și oferă raspunsuri în cazuri de repetate avorturi și de infertilitate idiopatică.


CaptureDe exemplu unui pacient ce prezintă un procent mai mare de 30% a fragmentării ADN-ului se recomandă folosirea Metodei de Inseminare Artificială.

Adițional cu Dynhalosperm aflăm cea mai bună calitate a spermei la un anumit timp și putem alege astfel momentul propice pentru o reproducere asistată .

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Sunt 3 tipuri de teste produse de Halotech Spania, ușor de folosit, fără ajutorul unui echipament sofisticat de laborator.

  • Halosperm ce pemite analiza fragmentării ADN-ului rapid și ușor
  • Dynhalosperm, singurul kit de pe piață ce masoară fragmentarea ADN-ului din spermă în timp
  • Halosperm Duo-vital ce este de ajutor pentru pacienții cu o motilitate a spermatozoizilor mai mică de 40%. Este o analiză suplimentară a numărului spematozoizilor.

Necrozoospermia - prezința unui numar mare a celulelor motile și non variabile - indică o patologie epidimală și un procent al celulelor vitale dar imotile ce prezintă defecte structurale ale flagelului.Detectează celulele cu o membrană intactă ce exclud moartea intracelulară. Astfel celulele vii apar verzi la un microscop cu fluorescență și cele moarte apar roșii. Rezultatele se masoară folosind un citometru de fluix pentru o analiză elaborata.




Sperm DNA Fragmentation Test for Veterinary Use Halomax® is an in vitro diagnostic solution that allows the measurement of sperm DNA fragmentation for every specific animal specie. Halomax® offers a better evaluation of semen quality compared to  a traditional method. Spermiogram fails taking into account the most important valuable parameter for a breeder, which is the quality of the genetic material that the sperm cell will transmit to the oocyte. Elevated sperm DNA fragmentation has a negative impact on pregnancy rates and litter size."
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>> Oxidative stress is essentially an imbalance between the production of free radicals and the body’s ability to counteract their potential negative effects. Blockage of oxidative stress is achieved through the action of antioxidant substances, which the body generates itself or which are administered externally.

Changes in the normal redox state of cells can cause cell toxicity, induced directly by the reactive oxygen, or from its derivatives like superoxide radicals, hydroxyl radicals or peroxides. These types of molecules can generate damage to all cell components–proteins, lipids, and DNA–if not offset by specific antioxidant action.

The human spermatozoon is highly susceptible to oxidative stress. Peroxidative damage directly affects the lipid component of the membrane and also generates breaks in the chains of both nuclear and mitochondrial DNA.

Applications of OxiSperm®

  • Oxisperm®, for its clinical application, is an easy-to-perform and reliable assay that allows the measurement of potential excess superoxide anions present in the ejaculate, with a minimum volume of sperm
  • Determination of oxidation levels in the ejaculate and reanalysis after treatment with antioxidants
  •  Evaluation of the oxidation in each patient to select the best sample that can be presented    <<

Vitaltest® allows the evaluation of the quality of the sperm membrane and the differentiation between live spermatozoa (intact membranes) and dead ones (affected membranes)

Rapid application, reliable, and low-cost technologies are essential for quantifying the state of the cell populations. The viability of spermatozoa in any ejaculate is related to the presence or absence of altered membranes.

Its use is advisable for patients with progressive motility below 40% to evaluate the proportion of live and dead spermatozoa. This test is used as a check on the motility evaluation, as the percentage of dead cells should not exceed that of immotile spermatozoa.

Cases of necrozoospermia are related to epididymal pathology (Wilton et al, 1988; Correa-Pérez et al, 2004). A high percentage of vital but immotile spermatozoa is indicative of structural defects in the flagellum (Chemes and Rawe, 2003).

Applications of Vitaltest®

  • Immediate determination* of the quality of the membrane of spermatozoa in the ejaculate.
  • Determination of necrozoospermia.
  • The incubation of sperm with the reagent is not necessary. <<

Halosperm is an in vitro diagnostic kit that allows the measurement of DNA fragmentation in a fast, easy, and reproducible manner, without the need for complex laboratory equipment. Halosperm allows for an improved evaluation of sperm quality, complementing the information offered by the conventional sperm analysis. Conventional semen analysis only studies spermatozoa concentration, motility and morphology.  It is an incomplete study, as it omits analysis of one of the most important parameters, the integrity of the DNA molecule. 15% of men considered to be infertile present normal sperm analyses, which makes a deeper assessment of the sample important. Halosperm facilitates decision-making for medical professionals regarding which assisted reproduction technique is most appropriate for each couple.

Halosperm is based on the SCD technique, patented by Halotech, based on a controlled DNA denaturation process to facilitate the subsequent removal of the proteins contained in each spermatozoon.  In this way, normal spermatozoa create halos formed by loops of DNA at the head of the sperm, which are not present in those with damaged DNA.

The images of halos generated with Halosperm® are highly contrasted and can be evaluated precisely using conventional or fluorescent microscopy. The tails of the spermatozoa are preserved, so it is possible to discriminate them from other cell types that might be present in the ejaculate, such as leukocytes. In addition, Halosperm® allows the visualisation of spermatozoa that contain highly degraded DNA compared to other types of less aggressive damage. This fact is of great importance in the diagnosis and monitoring of varicocele.

Medical professionals use information from Halotech solutions at the start of treatment, supplementing conventional semen analysis. Sperm DNA damage is a multifactorial process. Because it can have multiple causes, fragmentation, oxidation, and vitality analysis is especially recommended for:

  • Couples with a history of miscarriages.
  • Couples with unexplained infertility for more than 6 months
  • Men older than 40
  • Use of tight-fitting clothing
  • Men with a history of cancer
  • Men under treatment with prescription medications
  • Men exposed to toxic agents
  • Men who have had urogenital infections
  • Men with unhealthy lifestyle habits: those who smoke, are sedentary, have an unbalanced diet or are obese.
  • Poor quality of the embryo during second cycles of egg donation
  • Male factor of unknown cause

Natural factors like improper maturation or oxidative stress translate into failures during the production of spermatozoa in the testicle.

Halosperm G2® measures DNA fragmentation in a fast, easy way, similar to Halosperm, but also partially eliminates the bad odour generated by the lysis. By using a few drops, the test will offer the same result as the Halosperm test, maintaining its simplicity and ease of use.

Halosperm G2® also includes staining solutions for bright field microscopy. <<



>> The Fluorescence Microscopy Staining Kits reduce background noise and provide  higher contrast images with a DNA-specific fluorochrome for use with Halosperm®, Halosperm G2® and Halomax®.

Available in Green and Red. <<

Sursa:  www.halotechdna.com