Master mix Luna Universal Probe Q PCR Probe

Caracteristici standard:
Specificatii complete
Product Name Luna® Universal Probe qPCR Master Mix
Concentration:  2X Concentrate
Shelf Life: 24 months
Storage Temp: -20°C
Composition (1X) Proprietary
Specification Version: PS-M3004S/L/G v1.0
Effective Date: 01-Dec-16
Functional Testing (qPCR) - Luna® Universal Probe qPCR Master Mix is functionally tested in qPCR with human cDNA template, resulting in a standard curve with a calculated qPCR efficiency of 90-110%, and a dynamic range of 5 orders of magnitude.
Non-Specific DNase Activity (16 hour, Master Mix) - A 50 µl reaction in 1X Luna® Universal Probe qPCR Master Mix containing 1 µg of T3 DNA in addition to a reaction containing Lambda-HindIII DNA incubated for 16 hours at 37ºC results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis.
qPCR DNA Contamination (E. coli Genomic) - A minimum of 1 µl of Luna® Universal Probe qPCR Master Mix is screened for the presence of E. coli genomic DNA using SYBR® Green qPCR with primers specific for the E. coli 16S rRNA locus. Results are quantified using a standard curve generated from purified E. coli genomic DNA. The measured level of E. coli genomic DNA contamination is ζ 1 E. coli genome.
RNase Activity Assay (4 Hour Digestion) - A 10 µl reaction in NEBuffer 4 containing 40 ng of a 300 base single-stranded RNA and a minimum of 1 µl of Luna® Universal Probe qPCR Master Mix is incubated at 37ºC. After incubation for 4 hours, >90% of the substrate RNA remains intact as determined by gel electrophoresis using fluorescent detection.